Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab154555)

Overview

  • Product name
    Anti-Poly(A) RNA polymerase, mitochondrial antibody
    See all Poly(A) RNA polymerase, mitochondrial primary antibodies
  • Description
    Rabbit polyclonal to Poly(A) RNA polymerase, mitochondrial
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1 and 582 of Human Poly(A) RNA polymerase, mitochondrial (UniProt ID: Q9NVV4).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab154555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
  • Tissue specificity
    Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
  • Involvement in disease
    Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
  • Sequence similarities
    Belongs to the DNA polymerase type-B-like family.
    Contains 1 PAP-associated domain.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610027A18Rik antibody
    • AW551379 antibody
    • EC 2.7.7.19 antibody
    • FLJ10486 antibody
    • mitochondrial antibody
    • Mitochondrial poly(A) polymerase antibody
    • mtPAP antibody
    • PAP antibody
    • PAP-associated domain-containing protein 1 antibody
    • PAPD1 antibody
    • PAPD1_HUMAN antibody
    • Poly(A) RNA polymerase antibody
    • Poly(A) RNA polymerase, mitochondrial antibody
    • Poly(A) RNA polymerase, mitochondrial precursor antibody
    • Polynucleotide adenylyltransferase antibody
    • RP11-305E6.3 antibody
    • Terminal uridylyltransferase 1 antibody
    • TUTase 1 antibody
    see all

Images

  • Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab154555) at 1/5000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 66 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed Hela cell labeling Poly(A) RNA polymerase, mitochondrial with ab154555 at a 1/200 dilution. The lower panel was costained with Hoechst 33342.

References

ab154555 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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