Anti-MTPAP antibody (ab156684)
Key features and details
- Rabbit polyclonal to MTPAP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MTPAP antibody
See all MTPAP primary antibodies -
Description
Rabbit polyclonal to MTPAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human MTPAP aa 532-582.
Database link: NP_060579.3 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab156684 was affinity purified using an epitope specific to MTPAP, mitochondrial immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. -
Tissue specificity
Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle. -
Involvement in disease
Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105). -
Sequence similarities
Belongs to the DNA polymerase type-B-like family.
Contains 1 PAP-associated domain. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 450875 Chimpanzee
- Entrez Gene: 101132238 Gorilla
- Entrez Gene: 55149 Human
- Entrez Gene: 100452869 Orangutan
- Omim: 613669 Human
- SwissProt: Q9NVV4 Human
- Unigene: 173946 Human
-
Alternative names
- 0610027A18Rik antibody
- AW551379 antibody
- EC 2.7.7.19 antibody
see all
Images
-
All lanes : Anti-MTPAP antibody (ab156684) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 seconds -
Detection of MTPAP, mitochondrial by Western blot of Immunoprecipitate:
Immunoprecipitation of HeLa whole cell lysate using ab156684 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of MTPAP, mitochondrial with ab156684 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab156684 has not yet been referenced specifically in any publications.