Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab156684)

Overview

  • Product name
    Anti-Poly(A) RNA polymerase, mitochondrial antibody
    See all Poly(A) RNA polymerase, mitochondrial primary antibodies
  • Description
    Rabbit polyclonal to Poly(A) RNA polymerase, mitochondrial
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 532 and 582 of Human Poly(A) RNA polymerase, mitochondrial (NP_060579.3).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156684 was affinity purified using an epitope specific to Poly(A) RNA polymerase, mitochondrial immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
  • Tissue specificity
    Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
  • Involvement in disease
    Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
  • Sequence similarities
    Belongs to the DNA polymerase type-B-like family.
    Contains 1 PAP-associated domain.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610027A18Rik antibody
    • AW551379 antibody
    • EC 2.7.7.19 antibody
    • FLJ10486 antibody
    • mitochondrial antibody
    • Mitochondrial poly(A) polymerase antibody
    • mtPAP antibody
    • PAP antibody
    • PAP-associated domain-containing protein 1 antibody
    • PAPD1 antibody
    • PAPD1_HUMAN antibody
    • Poly(A) RNA polymerase antibody
    • Poly(A) RNA polymerase, mitochondrial antibody
    • Poly(A) RNA polymerase, mitochondrial precursor antibody
    • Polynucleotide adenylyltransferase antibody
    • RP11-305E6.3 antibody
    • Terminal uridylyltransferase 1 antibody
    • TUTase 1 antibody
    see all

Images

  • All lanes : Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab156684) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 30 seconds
  • Detection of Poly(A) RNA polymerase, mitochondrial by Western blot of Immunoprecipitate:
    Immunoprecipitation of HeLa whole cell lysate using ab156684 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of Poly(A) RNA polymerase, mitochondrial with ab156684 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab156684 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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