Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab78618)


  • Product nameAnti-Poly(A) RNA polymerase, mitochondrial antibody
    See all Poly(A) RNA polymerase, mitochondrial primary antibodies
  • Description
    Rabbit polyclonal to Poly(A) RNA polymerase, mitochondrial
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Poly(A) RNA polymerase, mitochondrial .

    (Peptide available as ab93076.)

  • Positive control
    • This antibody gave a positive signal in Human Skeletal Muscle Tissue Lysate; HepG2 Whole Cell Lysate and HEK293 Whole Cell Lysate.



Our Abpromise guarantee covers the use of ab78618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionPolymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
  • Tissue specificityUbiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
  • Involvement in diseaseDefects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
  • Sequence similaritiesBelongs to the DNA polymerase type-B-like family.
    Contains 1 PAP-associated domain.
  • Cellular localizationCytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610027A18Rik antibody
    • AW551379 antibody
    • EC antibody
    • FLJ10486 antibody
    • mitochondrial antibody
    • Mitochondrial poly(A) polymerase antibody
    • mtPAP antibody
    • PAP antibody
    • PAP-associated domain-containing protein 1 antibody
    • PAPD1 antibody
    • PAPD1_HUMAN antibody
    • Poly(A) RNA polymerase antibody
    • Poly(A) RNA polymerase, mitochondrial antibody
    • Poly(A) RNA polymerase, mitochondrial precursor antibody
    • Polynucleotide adenylyltransferase antibody
    • RP11-305E6.3 antibody
    • Terminal uridylyltransferase 1 antibody
    • TUTase 1 antibody
    see all

Anti-Poly(A) RNA polymerase, mitochondrial antibody images

  • All lanes : Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab78618) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,120 kDa,150 kDa,30 kDa,35 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab78618 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab78618 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab78618)

ab78618 has not yet been referenced specifically in any publications.

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