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Overview

  • Product nameAnti-Polycystin 1 antibody [7e12]
    See all Polycystin 1 primary antibodies
  • Description
    Mouse monoclonal [7e12] to Polycystin 1
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Bacterially expressed fusion protein from the N-terminal leucine rich domain of human Polycystin 1.

  • EpitopeThis antibody was produced to the flank-leucine rich repeat-flank region (24-180aa).
  • Positive control
    • This antibody gave a positive signal when used to probe a Human Liver Tissue section (IHC).

Properties

Applications

Our Abpromise guarantee covers the use of ab74115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
  • Application notesIs unsuitable for WB.

    • Target

    • FunctionMay be an ion-channel regulator. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Involved in adhesive protein-protein and protein-carbohydrate interactions.
    • Involvement in diseaseDefects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1) [MIM:173900]. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000.
    • Sequence similaritiesBelongs to the polycystin family.
      Contains 1 C-type lectin domain.
      Contains 1 GPS domain.
      Contains 1 LDL-receptor class A domain.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 17 PKD domains.
      Contains 1 PLAT domain.
      Contains 1 REJ domain.
      Contains 1 WSC domain.
    • DomainThe LDL-receptor class A domain is atypical; the potential calcium-binding site is missing.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Autosomal dominant polycystic kidney disease 1 protein antibody
      • Autosomal dominant polycystic kidney disease protein 1 antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • OTTHUMP00000208669 antibody
      • OTTHUMP00000208670 antibody
      • PBP antibody
      • Pc-1 antibody
      • PKD antibody
      • Pkd1 antibody
      • PKD1_HUMAN antibody
      • Polycystic Kidney Disease 1 antibody
      • polycystic kidney disease-associated protein antibody
      • Polycystin 1 Precursor antibody
      • Polycystin-1 antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Serine/threonine-protein kinase D1 antibody
      • transient receptor potential cation channel, subfamily P, member 1 antibody
      • TRPP1 antibody
      see all

    Anti-Polycystin 1 antibody [7e12] images

    • Immunocytochemistry/ Immunofluorescence - Polycystin 1 antibody [7e12] (ab74115)
      Immunocytochemistry/ Immunofluorescence - Polycystin 1 antibody [7e12] (ab74115)
      ICC/IF image of ab74115 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74115, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Polycystin 1 antibody [7e12] (ab74115)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Polycystin 1 antibody [7e12] (ab74115)
      IHC image of Polycystin 1 staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • Flow Cytometry-Anti-Polycystin 1 antibody [7e12](ab74115)
      Flow Cytometry-Anti-Polycystin 1 antibody [7e12](ab74115)

      Overlay histogram showing HEK293 cells stained with ab74115 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74115, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References for Anti-Polycystin 1 antibody [7e12] (ab74115)

    This product has been referenced in:
    • Aguiari G  et al. Novel role for polycystin-1 in modulating cell proliferation through calcium oscillations in kidney cells. Cell Prolif 41:554-73 (2008). Read more (PubMed: 18422703) »
    • Xu C  et al. Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling. Am J Physiol Renal Physiol 292:F930-45 (2007). Read more (PubMed: 17090781) »

    See all 8 Publications for this product

    Publishing research using ab74115? Please let us know so that we can cite the reference in this datasheet.

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    1 Abreview or Q&A

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    Western blot abreview for Anti-Polycystin 1 antibody [7e12]

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Dog Cell lysate - whole cell (kidney nephron)
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (6)
    Loading amount 300 µg
    Specification kidney nephron
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
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    Username

    Herr Dr. Andre Kraus

    Verified customer

    Submitted Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"