Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)

Overview

  • Product nameAnti-Polyethylene glycol antibody [26A04] - BSA and Azide free
    See all Polyethylene glycol primary antibodies
  • Description
    Rat monoclonal [26A04] to Polyethylene glycol - BSA and Azide free
  • Specificityab94764 recognizes linear 20 kDa PEG, linear 5 kDa PEG, linear 600 Da PEG and 10 kDa branched PEG. It also binds to BSA conjugated PEG by western blot.
  • Tested applicationsSuitable for: IHC-P, WB, Indirect ELISAmore details
  • Immunogen

    5 kDa PEG conjugated to KLH by a MBS linker.

  • Positive control
    • PEGylated protein
  • General notes

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
    when performing ELISA using anti-PEG products.

     

    Learn more about PEG RabMAb primary antibodies and kit - PEG RabMAb Product Portal Page

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituent: Tris buffered saline
  • Concentration information loading...
  • Purification notesCeramic hydroxyapatite (CHT) chromatography from in vitro production.
  • ClonalityMonoclonal
  • Clone number26A04
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
Indirect ELISA Use a concentration of 2 µg/ml.

Target

  • Alternative names
    • PEG antibody

Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free images

  • Indirect ELISA analysis of a PEG panel using ab94764.
  • Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764) + PEGylated protein

    Predicted band size : 1 kDa

References for Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)

This product has been referenced in:
  • Burtea C  et al. Screening for peptides targeted to IL-7Ra for molecular imaging of rheumatoid arthritis synovium. Arthritis Res Ther 18:230 (2016). Read more (PubMed: 27729062) »

See 1 Publication for this product

Product Wall

To avoid Tween20 and other PEG-containing detergents, I recommend antigen retrieval buffers http://www.abcam.com/100x-citrate-buffer-ph-60-ab64236.html (100x Citrate Buffer pH 6.0, 50mL) or http://www.abcam.com/10x-citrate-buffer-ph-60-ab64214.html (10...

Read More

Thank you for your enquiry and your interest.



I can confirm that we have determined that the specificity of the antibody (ab94764: Anti-Polyethylene glycol antibody) is to the linear backbone of PEG and that it will not recognize t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Atheroma on aorta of ApoE-KO mice)
Specification Atheroma on aorta of ApoE-KO mice
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - baths in 0.1% Tween-20 diluted in PBS
Blocking step Protein-Free Blocking Buffer, Pierce, Cat N° 37570 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2012

Thank you very much for your valuable feedback. We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative,...

Read More

Thank you for your reply. If the secondary antibody is directed against the heavy and light chains (H+L) of rat IgG, you will still be able to observe specific signal because the light chains are conserved between isotypes. If the secondary antibody is...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"