SpecificityThis antibody recognizes native as well as denatured, reduced forms of
synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml
Storage instructionsShipped at 4°C. Store at +4°C.
Primary antibody notesMonoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
RelevanceRecombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to
interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.
References for Anti-polyHistidine antibody [HIS-1] (HRP) (ab49781)
has not yet been referenced specifically in any publications.
Publishing research using ab49781? Please let us know so that we can cite the reference in this datasheet.
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