Anti-polyHistidine antibody [HIS-1] (HRP) (ab49781)

Overview

  • Product nameAnti-polyHistidine antibody [HIS-1] (HRP)
    See all polyHistidine primary antibodies
  • Description
    Mouse monoclonal [HIS-1] to polyHistidine (HRP)
  • ConjugationHRP
  • SpecificityThis antibody recognizes native as well as denatured, reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • Tested applicationsSuitable for: ELISA, Dot blot, WBmore details
  • Immunogen

    Recombinant polyHistidine tagged fusion protein.

  • Positive control
    • Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
  • General notes

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

    The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesMonoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
  • ClonalityMonoclonal
  • Clone numberHIS-1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Dot blot 1/2000.
WB 1/2000.

Target

  • RelevanceRecombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

References for Anti-polyHistidine antibody [HIS-1] (HRP) (ab49781)

ab49781 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"