Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085)

Overview

  • Product nameAnti-Polyoma virus, Medium T antigen antibody [PyMT]
  • Description
    Rat monoclonal [PyMT] to Polyoma virus, Medium T antigen
  • Tested applicationsSuitable for: IP, ICC/IF, WBmore details
  • Immunogen

    Synthetic peptide (N terminal).

  • General notes


    Binds medium T antigen only, allows isolation of viral T antigens.

Properties

Applications

Our Abpromise guarantee covers the use of ab15085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Target

  • RelevanceMiddle T antigen (MT) is a 421-amino-acid protein associated with membranes and underlying cytoskeletal elements, and is associated with a tyrosine-specific protein kinase activity. It is the principal oncoprotein of polyomavirus that is necessary and often sufficient for transformation in vitro. MT delivered as a transgene or a retrovirus can induce tumors in a wide variety of tissues. Polyomavirus (PyV) is a small, double-stranded, closed-circular-DNA virus with an approximately 5-kb genome divided into two roughly equal regions. The late transcripts produce the viral capsid proteins, whereas the early region encodes three so-called tumor (T) antigens that are important for both productive infection and transformation.
  • Cellular localizationCytoplasmic location in cells infected with virus.
  • Alternative names
    • Middle T antigen antibody
    • MT AG antibody
    • MT antibody
    • Polyoma virus middle t antigen antibody

References for Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085)

This product has been referenced in:
  • Dilworth SM & Griffin BE Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci U S A 79:1059-63 (1982). Read more (PubMed: 6175960) »
  • Dilworth SM Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen. EMBO J 1:1319-28 (1982). Read more (PubMed: 6327264) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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I am sorry for the delay in getting back to you in regards to the protocol used with the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085).
I can now provide some further guidance in how I would suggest using the antibody in IHC. From...

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Thank you for contacting us yesterday in regards to the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085).
Unfortunately, as we discussed over the phone, this antibody has as yet not been used in IHC to our knowledge. This does not me...

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Thank you very much for your patience.
We can confirm that the antibody is specific to Medium T form only. We have no cross reactivity data regarding small and large T forms. The clone specific publication data show the antibody should not be cross...

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Thank you for contacting us.

I am afraid ab15085 has not been tested in IHC, and therefore we cannot guarantee it will work in this application. You may find useful our online protocols about IHC in order to optimise the staining:

h...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Breast tumor)
Loading amount 50 µg
Specification Breast tumor
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jan 27 2011

Thank you for your email and patience. I presently have concerns about the suitability of this antibody in Western blotting and have removed the application from our online datasheet. We haven't received any other complaints, but the originator of this...

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Thank you for your enquiry. More details about this antibody can be found in the following references: Dilworth SM & Griffin BE Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci U S A 79:1059-63 (1982). PubMed: 6175960 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"