Overview

  • Product nameAnti-POMC antibody
    See all POMC primary antibodies
  • Description
    Goat polyclonal to POMC
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    C-NAIIKNAYKKGE

    , corresponding to C terminal amino acids 256-267 of Human POMC.

  • Positive control
    • Human lymph node and murine pituitary cell line NIH 3T3 lysates. Human adrenal gland tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).

This product appears to react with the full length precursor protein only.

IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/75. PubMed: 23469282
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • alpha melanocyte stimulating hormone antibody
    • Alpha MSH antibody
    • Alpha-MSH antibody
    • beta endorphin antibody
    • Beta LPH antibody
    • beta melanocyte stimulating hormone antibody
    • Beta MSH antibody
    • Beta-LPH antibody
    • Beta-MSH antibody
    • CLIP antibody
    • COLI_HUMAN antibody
    • Corticotropin like intermediary peptide antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-lipotropin antibody
    • Gamma MSH antibody
    • Gamma-LPH antibody
    • Gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • LPH antibody
    • Melanotropin alpha antibody
    • Melanotropin antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • met enkephalin antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • POC antibody
    • POMC antibody
    • pro ACTH endorphin antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    see all

Anti-POMC antibody images

  • ab32893 (4µg/ml) staining of POMC in paraffin embedded Human Adrenal Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows vesiculate staining in some glandular cells.

  • Anti-POMC antibody (ab32893) at 0.01 µg/ml + NIH3T3 cell lysate (35µg in RIPA buffer)

    Predicted band size : 29 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.
    Detected by chemiluminescence.
  • ab32893 at 2.5ug/ml staining of paraffin embedded human adrenal gland tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab32893 (4µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows vesiculate staining in some glandular cells.

References for Anti-POMC antibody (ab32893)

This product has been referenced in:
  • Marco A  et al. DNA CpG Methylation (5-Methylcytosine) and Its Derivative (5-Hydroxymethylcytosine) Alter Histone Posttranslational Modifications at the Pomc Promoter, Affecting the Impact of Perinatal Diet on Leanness and Obesity of the Offspring. Diabetes 65:2258-67 (2016). Read more (PubMed: 27217481) »
  • Carlson RI  et al. Development and application of an antibody-based protein microarray to assess physiological stress in grizzly bears (Ursus arctos). Conserv Physiol 4:cow001 (2016). Other . Read more (PubMed: 27293753) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal gland)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization No
Specification Adrenal gland
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% Normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Dec 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Dec 06 2016

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