• Product name
  • Description
    Rabbit polyclonal to POMC
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Rat POMC aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P01194

  • Positive control
    • This antibody gave a positive signal in PC12 whole cell lysate as well as Mouse and Rat Brain tissue lysates. This antibody gave a positive result in IHC in the following frozen tissue: Rat Brain (Arcuate Nucleus of the Hypothalamus).



Our Abpromise guarantee covers the use of ab94446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Information by UniProt
  • Database links
  • Alternative names
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • alpha melanocyte stimulating hormone antibody
    • Alpha MSH antibody
    • Alpha-MSH antibody
    • beta endorphin antibody
    • Beta LPH antibody
    • beta melanocyte stimulating hormone antibody
    • Beta MSH antibody
    • Beta-LPH antibody
    • Beta-MSH antibody
    • CLIP antibody
    • COLI_HUMAN antibody
    • Corticotropin like intermediary peptide antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-lipotropin antibody
    • Gamma MSH antibody
    • Gamma-LPH antibody
    • Gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • LPH antibody
    • Melanotropin alpha antibody
    • Melanotropin antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • met enkephalin antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • POC antibody
    • POMC antibody
    • pro ACTH endorphin antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    see all


  • IHC image of POMC staining in Rat Brain (Arcuate Nucleus of the Hypothalamus) formalin fixed frozen tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94446, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-POMC antibody (ab94446) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 30 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94446 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


This product has been referenced in:
  • Xu G  et al. Cyclocarya paliurus (Batal.) Ijinskaja Aqueous Extract (CPAE) Ameliorates Obesity by Improving Insulin Signaling in the Hypothalamus of a Metabolic Syndrome Rat Model. Evid Based Complement Alternat Med 2017:4602153 (2017). Read more (PubMed: 28684967) »
  • Rahman TU  et al. Hypertriglyceridemia in female rats during pregnancy induces obesity in male offspring via altering hypothalamic leptin signaling. Oncotarget 8:53450-53464 (2017). Read more (PubMed: 28881823) »

See all 2 Publications for this product

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