Mouse, Rat Predicted to work with:
Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Rat POMC aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH). Database link: P01194
This antibody gave a positive signal in PC12 whole cell lysate as well as Mouse and Rat Brain tissue lysates.
This antibody gave a positive result in IHC in the following frozen tissue:
Rat Brain (Arcuate Nucleus of the Hypothalamus).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
IHC image of POMC staining in Rat Brain (Arcuate Nucleus of the Hypothalamus) formalin fixed frozen tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94446, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-POMC antibody (ab94446)
All lanes : Anti-POMC antibody (ab94446) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa Observed band size : 26 kDa
Exposure time : 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94446 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Xu G et al. Cyclocarya paliurus (Batal.) Ijinskaja Aqueous Extract (CPAE) Ameliorates Obesity by Improving Insulin Signaling in the Hypothalamus of a Metabolic Syndrome Rat Model. Evid Based Complement Alternat Med2017:4602153 (2017).
Read more (PubMed: 28684967) »