• Product nameAnti-POMT1 antibody
    See all POMT1 primary antibodies
  • Description
    Rabbit polyclonal to POMT1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
  • Immunogen

    A synthetic peptide corresponding to a region within the internal sequence amino acids 647-696 (LTFQILLLPV VLQHISDHLC RSQLQRSIFS ALVVAWYSSA CHVSNTLRPL) of Human POMT1 (NP_001070833).

  • Positive control
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab83558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31, 85 kDa (predicted molecular weight: 85 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.


  • FunctionTransfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
  • Tissue specificityWidely expressed. Highly expressed in testis, heart and pancreas. Detected at lower levels in kidney, skeletal muscle, brain, placenta, lung and liver.
  • PathwayProtein modification; protein glycosylation.
  • Involvement in diseaseDefects in POMT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with mental retardation type B1 (MDDGB1) [MIM:613155]; also called muscular dystrophy congenital POMT1-related. MDDGB1 is an autosomal recessive disorder characterized by congenital muscular dystrophy associated with mental retardation and mild structural brain abnormalities.
    Defects in POMT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A1 (MDDGA1) [MIM:236670]; also known as hydrocephalus-agyria-retinal dysplasia or HARD syndrome. MDDGA1 is an autosomal recessive disorder characterized by cobblestone lissencephaly, hydrocephalus, agyria, retinal displasia, with or without encephalocele. It is often associated with congenital muscular dystrophy and usually lethal within the first few months of life. Included diseases are the more severe Walker-Warburg syndrome and the slightly less severe muscle-eye-brain disease.
    Defects in POMT1 are the cause of muscular dystrophy-dystroglycanopathy limb-girdle type C1 (MDDGC1) [MIM:609308]; also called autosomal recessive limb-girdle muscular dystrophy with mental retardation. MDDGC1 is a novel form of recessive limb girdle muscular dystrophy with mild mental retardation without any obvious structural brain abnormality, associated with an abnormal alpha-dystroglycan pattern in the muscle. MDDGC1 is a significantly milder allelic form of WWS.
  • Sequence similaritiesBelongs to the glycosyltransferase 39 family.
    Contains 3 MIR domains.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dolichyl phosphate mannose protein mannosyltransferase 1 antibody
    • Dolichyl phosphate mannose protein mannosyltransferase1 antibody
    • Dolichyl-phosphate-mannose--protein mannosyltransferase 1 antibody
    • LGMD2K antibody
    • MDDGA1 antibody
    • MDDGB1 antibody
    • MDDGC1 antibody
    • POMT 1 antibody
    • POMT1 antibody
    • POMT1_HUMAN antibody
    • Protein O mannosyl transferase 1 antibody
    • Protein O mannosyl transferase1 antibody
    • Protein O-mannosyl-transferase 1 antibody
    • Rotate abdomen, Drosophila, homolog of antibody
    • RT antibody
    see all

Anti-POMT1 antibody images

  • Anti-POMT1 antibody (ab83558) at 1 µg/ml (in 5% skim milk/ PBS) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 31,85 kDa (why is the actual band size different from the predicted?)

References for Anti-POMT1 antibody (ab83558)

ab83558 has not yet been referenced specifically in any publications.

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