Recombinant
RabMAb

Anti-POMT1 antibody [EPR11680] (ab175234)

Overview

  • Product name
    Anti-POMT1 antibody [EPR11680]
    See all POMT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11680] to POMT1
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human POMT1 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y6A1

  • Positive control
    • Human fetal heart, HepG2, BxPC-3 and Human testis lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab175234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 85 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
    • Tissue specificity
      Widely expressed. Highly expressed in testis, heart and pancreas. Detected at lower levels in kidney, skeletal muscle, brain, placenta, lung and liver.
    • Pathway
      Protein modification; protein glycosylation.
    • Involvement in disease
      Defects in POMT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with mental retardation type B1 (MDDGB1) [MIM:613155]; also called muscular dystrophy congenital POMT1-related. MDDGB1 is an autosomal recessive disorder characterized by congenital muscular dystrophy associated with mental retardation and mild structural brain abnormalities.
      Defects in POMT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A1 (MDDGA1) [MIM:236670]; also known as hydrocephalus-agyria-retinal dysplasia or HARD syndrome. MDDGA1 is an autosomal recessive disorder characterized by cobblestone lissencephaly, hydrocephalus, agyria, retinal displasia, with or without encephalocele. It is often associated with congenital muscular dystrophy and usually lethal within the first few months of life. Included diseases are the more severe Walker-Warburg syndrome and the slightly less severe muscle-eye-brain disease.
      Defects in POMT1 are the cause of muscular dystrophy-dystroglycanopathy limb-girdle type C1 (MDDGC1) [MIM:609308]; also called autosomal recessive limb-girdle muscular dystrophy with mental retardation. MDDGC1 is a novel form of recessive limb girdle muscular dystrophy with mild mental retardation without any obvious structural brain abnormality, associated with an abnormal alpha-dystroglycan pattern in the muscle. MDDGC1 is a significantly milder allelic form of WWS.
    • Sequence similarities
      Belongs to the glycosyltransferase 39 family.
      Contains 3 MIR domains.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dolichyl phosphate mannose protein mannosyltransferase 1 antibody
      • Dolichyl phosphate mannose protein mannosyltransferase1 antibody
      • Dolichyl-phosphate-mannose--protein mannosyltransferase 1 antibody
      • LGMD2K antibody
      • MDDGA1 antibody
      • MDDGB1 antibody
      • MDDGC1 antibody
      • POMT 1 antibody
      • POMT1 antibody
      • POMT1_HUMAN antibody
      • Protein O mannosyl transferase 1 antibody
      • Protein O mannosyl transferase1 antibody
      • Protein O-mannosyl-transferase 1 antibody
      • Rotate abdomen, Drosophila, homolog of antibody
      • RT antibody
      see all

    Images

    • All lanes : Anti-POMT1 antibody [EPR11680] (ab175234) at 1/10000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : HepG2 lysate
      Lane 3 : BxPC-3 lysate
      Lane 4 : Human testis lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 85 kDa
    • Immunoprecipitation of POMT1 from Human fetal heart lysate using ab175234 at 1/10.

    References

    ab175234 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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