Overview

  • Product nameAnti-POMT2 antibody
    See all POMT2 primary antibodies
  • Description
    Rabbit polyclonal to POMT2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human POMT2 (NP_037514)

  • Positive control
    • HeLa cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab76396 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab76396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionTransfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
  • Tissue specificityHighly expressed in testis; detected at low levels in most tissues.
  • PathwayProtein modification; protein glycosylation.
  • Involvement in diseaseMuscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A2 (MDDGA2) [MIM:613150]: An autosomal recessive disorder characterized by congenital muscular dystrophy associated with cobblestone lissencephaly and other brain anomalies, eye malformations, profound mental retardation, and death usually in the first years of life. Included diseases are the more severe Walker-Warburg syndrome and the slightly less severe muscle-eye-brain disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Muscular dystrophy-dystroglycanopathy congenital with mental retardation B2 (MDDGB2) [MIM:613156]: An autosomal recessive disorder characterized by congenital muscular dystrophy associated with mental retardation and mild structural brain abnormalities. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Muscular dystrophy-dystroglycanopathy limb-girdle C2 (MDDGC2) [MIM:613158]: An autosomal recessive muscular dystrophy with onset after ambulation is achieved. MDDGC2 is characterized by increased serum creatine kinase and mild muscle weakness. Muscle biopsy shows dystrophic changes, inflammatory changes, and severely decreased alpha-dystroglycan. Cognition is normal. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesBelongs to the glycosyltransferase 39 family.
    Contains 3 MIR domains.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830009D15Rik antibody
    • AW046274 antibody
    • DKFZp686G10254 antibody
    • Dolichyl phosphate mannose protein mannosyltransferase 2 antibody
    • Dolichyl-phosphate-mannose--protein mannosyltransferase 2 antibody
    • FLJ22309 antibody
    • LGMD2N antibody
    • MDDGA2 antibody
    • MDDGB2 antibody
    • MDDGC2 antibody
    • POMT 2 antibody
    • Pomt2 antibody
    • POMT2_HUMAN antibody
    • Protein O mannosyltransferase 2 antibody
    • Protein O mannosyltransferase antibody
    • Protein O-mannosyl-transferase 2 antibody
    • Putative protein O mannosyltransferase antibody
    • rCG_20643 antibody
    see all

Anti-POMT2 antibody images

  • Anti-POMT2 antibody (ab76396) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : 84 kDa

References for Anti-POMT2 antibody (ab76396)

ab76396 has not yet been referenced specifically in any publications.

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