Recombinant
RabMAb

Anti-PON1 antibody [EPR2892] (ab92466)

Overview

  • Product name
    Anti-PON1 antibody [EPR2892]
    See all PON1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2892] to PON1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human PON1.

  • Positive control
    • WB: plasma cells and ovary carcinoma lysate; IHC-P: liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab92466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    IP: 1/10 - 1/100.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.

    Is unsuitable for ICC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
    • Tissue specificity
      Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
    • Involvement in disease
      Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
    • Sequence similarities
      Belongs to the paraoxonase family.
    • Post-translational
      modifications
      Glycosylated.
      The signal sequence is not cleaved.
      Present in two forms, form B contains a disulfide bond, form A does not.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • A esterase 1 antibody
      • A-esterase 1 antibody
      • Aromatic esterase 1 antibody
      • Arylesterase 1 antibody
      • Arylesterase B type antibody
      • ESA antibody
      • Esterase A antibody
      • K 45 antibody
      • K-45 antibody
      • MVCD5 antibody
      • Paraoxonase 1 antibody
      • Paraoxonase antibody
      • Paraoxonase B type antibody
      • Paraoxonase, plasma antibody
      • Paraoxonase1 antibody
      • PON 1 antibody
      • PON antibody
      • PON1 antibody
      • PON1_HUMAN antibody
      • Serum aryldiakylphosphatase antibody
      • Serum aryldialkylphosphatase 1 antibody
      • Serum paraoxonase/arylesterase 1 antibody
      see all

    Images

    • All lanes : Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution

      Lane 1 : Human plasma cell lysate
      Lane 2 : ovarian cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 40 kDa
    • ab92466, at 1/100 dilution, staining PON1 in formalin-fixed, paraffin-embedded Human liver tissue.
    • ab92466 showing negative staining in Skeletal muscle tissue.

    • ab92466 showing negative staining in Normal colon tissue.

    • ab92466 showing negative staining in Normal breast tissue.

    • ab92466 showing negative staining in Normal brain tissue.

    References

    ab92466 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your patience. I have received some confirmation about some products and would like to update you on the information. ab85347: We have not tested this product for cross reactivity with PON2 and PON3. ab40775: We have not teste...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up