Recombinant Anti-PON1 antibody [EPR2893] (ab126597)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2893] to PON1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-PON1 antibody [EPR2893]
See all PON1 primary antibodies -
Description
Rabbit monoclonal [EPR2893] to PON1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PON1 aa 100-200. The exact sequence is proprietary.
-
Positive control
- IP: Human liver lysate. WB: Human mouse and rat plasma and liver lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2893 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/20.
|
|
WB |
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
|
Notes |
---|
IP
1/20. |
WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Target
-
Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. -
Tissue specificity
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. -
Involvement in disease
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsGlycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 5444 Human
- Entrez Gene: 18979 Mouse
- Entrez Gene: 84024 Rat
- Omim: 168820 Human
- SwissProt: P27169 Human
- SwissProt: P52430 Mouse
- SwissProt: P55159 Rat
- Unigene: 370995 Human
see all -
Alternative names
- A esterase 1 antibody
- A-esterase 1 antibody
- Aromatic esterase 1 antibody
see all
Images
-
All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human liver lysate
Lane 3 : Mouse plasma lysate
Lane 4 : Mouse liver lysate
Lane 5 : Rat plasma lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?The expression profile and molecular weights observed are consistent with what have been described in the literatures (PMID: 17906223, 30262871).
-
Purified ab126597 at 1/20 dilution (0.5µg) immunoprecipitating PON1 in Human liver lysate.
Lane 1 (input): Human liver lysate 10µg
Lane 2 (+): ab126597 + Human liver lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126597 in Human liver lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 40-45 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab126597 has been referenced in 3 publications.
- Zhang L et al. Pon1 Deficiency Promotes Trem2 Pathway-Mediated Microglial Phagocytosis and Inhibits Pro-inflammatory Cytokines Release In Vitro and In Vivo. Mol Neurobiol 59:4612-4629 (2022). PubMed: 35589918
- Li Y et al. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis. Arterioscler Thromb Vasc Biol 39:1574-1587 (2019). PubMed: 31291759
- Huang Y et al. Myeloperoxidase, paraoxonase-1, and HDL form a functional ternary complex. J Clin Invest 123:3815-28 (2013). IP ; Human . PubMed: 23908111