Recombinant
RabMAb

Anti-PON2 antibody [EPR15295] (ab183710)

Overview

  • Product name
    Anti-PON2 antibody [EPR15295]
    See all PON2 primary antibodies
  • Description
    Rabbit monoclonal [EPR15295] to PON2
  • Specificity
    Based on the sequence analysis, ab183710 recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PON2 aa 200-350. The exact sequence is proprietary.
    Database link: Q15165

  • Positive control
    • HeLa, HepG2, A431 and U87-MG cell lysates; Human and Mouse kidney tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/100.
IP 1/50 - 1/70.

Target

  • Function
    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
  • Tissue specificity
    Widely expressed with highest expression in liver, lung, placenta, testis and heart.
  • Sequence similarities
    Belongs to the paraoxonase family.
  • Post-translational
    modifications
    The signal sequence is not cleaved.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A esterase 2 antibody
    • A-esterase 2 antibody
    • Aromatic esterase 2 antibody
    • Paraoxonase 2 antibody
    • Paraoxonase nirs antibody
    • PON 2 antibody
    • PON2 antibody
    • PON2_HUMAN antibody
    • Serum aryldialkylphosphatase 2 antibody
    • Serum paraoxonase/arylesterase 2 antibody
    see all

Images

  • All lanes : Anti-PON2 antibody [EPR15295] (ab183710) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39,41 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2: TBS instead of HepG2 lysates.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab183710 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Human Tissue (normal and tumor sections)
Specification
normal and tumor sections
Username

Abcam user community

Verified customer

Submitted Feb 27 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T, mouse embryonic fibroblasts)
Specification
293T, mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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