Overview

  • Product nameAnti-PON3 antibody
    See all PON3 primary antibodies
  • Description
    Rabbit polyclonal to PON3
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON3.

    (Peptide available as ab42321.)

  • Positive control
    • WB: Human skeletal muscle, mouse liver, rat liver and rat skeletal muscle tissue lysates. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab42322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).

Target

Anti-PON3 antibody images

  • ICC/IF image of ab42322 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PON3 antibody (ab42322) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab42322 staining PON3 in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42322, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PON3 antibody (ab42322)

This product has been referenced in:
  • Pei JF  et al. Human paraoxonase gene cluster overexpression alleviates angiotensin II-induced cardiac hypertrophy in mice. Sci China Life Sci 59:1115-1122 (2016). Read more (PubMed: 27578362) »
  • Stone R  et al. Identification of genes correlated with early-stage bladder cancer progression. Cancer Prev Res (Phila Pa) 3:776-86 (2010). IHC-P ; Mouse . Read more (PubMed: 20501863) »

See all 2 Publications for this product

Product Wall

The PON3 antibody that I would like to send to you free-of-charge is ab108964. This is a rabbit monoclonal that has given good western blot results for a variety of human tissue and cell lysates. However, it is not suitable for IHC or ICC.

C...

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Thank you for your email and the additional information. This is very helpful.

I would suggest that if no protease inhibitors were used, to include them in your lysis buffer freshly, before lysing the cells (e.g. ab65621 protease Inhibitor c...

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Thank you for bringing this to our attention.

I am sorry to read that you are having difficulty obtaining a western blot signal. I may be able to make some recommendations but I will need a few more details about your samples and protocol. <...

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