Anti-PON3 antibody [EPR2902(2)] (ab108964)

Overview

  • Product nameAnti-PON3 antibody [EPR2902(2)]
    See all PON3 primary antibodies
  • Description
    Rabbit monoclonal [EPR2902(2)] to PON3
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PON3.

  • Positive control
    • Human fetal liver, A549, HepG2, Mouse kidney, and Mouse liver lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionHas low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
    • Sequence similaritiesBelongs to the paraoxonase family.
    • Post-translational
      modifications
      The signal sequence is not cleaved.
    • Cellular localizationSecreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810004E20 antibody
      • AI786302 antibody
      • MGC95026 antibody
      • Paraoxonase 3 antibody
      • Pon3 antibody
      • PON3_HUMAN antibody
      • Serum paraoxonase/lactonase 3 antibody
      see all

    Anti-PON3 antibody [EPR2902(2)] images

    • All lanes : Anti-PON3 antibody [EPR2902(2)] (ab108964) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : A549 lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa

    References for Anti-PON3 antibody [EPR2902(2)] (ab108964)

    ab108964 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (rat intestinal epithelial cells (RIE-1))
    Permeabilization Yes - 0.2% TritonX-100
    Specification rat intestinal epithelial cells (RIE-1)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 03 2016

    Application Flow Cytometry
    Sample Mouse Cell (Mouse Lewis Lung Carcinoma cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification Mouse Lewis Lung Carcinoma cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 22 2016

    Application IHC - Wholemount
    Sample Mouse Tissue (Transplanted lung tumor section)
    Specification Transplanted lung tumor section
    Username

    Abcam user community

    Verified customer

    Submitted Dec 18 2015

    Application IHC - Wholemount
    Sample Human Tissue (human ovary adenocarcinoma)
    Specification human ovary adenocarcinoma
    Username

    Abcam user community

    Verified customer

    Submitted Jun 03 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549 lung cancer cells)
    Permeabilization Yes - 0.2% Triton X-100
    Specification A549 lung cancer cells
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 07 2015

    Application Flow Cytometry
    Sample Human Cell (A549 lung cancer cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification A549 lung cancer cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 29 2015

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-20%)
    Sample Human Cell lysate - whole cell (HEK293T cellls)
    Specification HEK293T cellls
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Chi Li

    Verified customer

    Submitted Dec 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"