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Rabbit polyclonal to POP1
Predicted to work with:
Mouse, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide, corresponding to a region between residues 75 and 125 of human POP1 (NP_055844.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab85757 was affinity purified using an epitope specific to POP1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
Use at 5-10 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POP1 antibody (ab85757)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling POP1 with ab85757 at 1/200 (1µg/ml). Detection: DAB.
Western blot - POP1 antibody (ab85757)
All lanes :
Anti-POP1 antibody (ab85757) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - POP1 antibody (ab85757)
Detection of POP1 by Western Blot of Immunprecipitate.
ab85757 at 0.4µg/ml staining POP1 in HeLa whole cell lysate immunoprecipitated using ab85757 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"