Overview

  • Product nameAnti-POP1 antibody
    See all POP1 primary antibodies
  • Description
    Rabbit polyclonal to POP1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 75 and 125 of human POP1 (NP_055844.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionComponent of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4932434G09Rik antibody
    • hPOP1 antibody
    • KIAA0061 antibody
    • MGC17365 antibody
    • MGC36536 antibody
    • POP1 antibody
    • POP1_HUMAN antibody
    • Processing of precursor 1 antibody
    • Processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae) antibody
    • Processing of precursors 1 antibody
    • Ribonucleases P/MRP protein subunit homolog antibody
    • Ribonucleases P/MRP protein subunit POP1 antibody
    see all

Anti-POP1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling POP1 with ab85757 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-POP1 antibody (ab85757) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 115 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of POP1 by Western Blot of Immunprecipitate.
    ab85757 at 0.4µg/ml staining POP1 in HeLa whole cell lysate immunoprecipitated using ab85757 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-POP1 antibody (ab85757)

ab85757 has not yet been referenced specifically in any publications.

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