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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab85757 was affinity purified using an epitope specific to POP1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
Use at 5-10 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Nucleus > nucleolus.
Information by UniProt
Anti-POP1 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POP1 antibody (ab85757)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling POP1 with ab85757 at 1/200 (1µg/ml). Detection: DAB.
Western blot - POP1 antibody (ab85757)
All lanes : Anti-POP1 antibody (ab85757) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size : 115 kDa Observed band size : 120 kDa ( why is the actual band size different from the predicted?) Exposure time : 3 minutes
Immunoprecipitation - POP1 antibody (ab85757)
Detection of POP1 by Western Blot of Immunprecipitate.
ab85757 at 0.4µg/ml staining POP1 in HeLa whole cell lysate immunoprecipitated using ab85757 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-POP1 antibody (ab85757)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"