Overview

  • Product nameAnti-POPDC2 antibody
  • Description
    Mouse polyclonal to POPDC2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human POPDC2 protein (NP_071418).

  • Positive control
    • POPDC2 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).

Target

  • RelevancePOPDC2 is a member of the POP family of proteins which contain three putative transmembrane domains. POPDC2 is predominantly expressed in skeletal and cardiac muscle and may play an important role in heart development.
  • Cellular localizationMembrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • POP 2 antibody
    • POP2 antibody
    • POPDC 2 antibody
    • Popeye domain containing 2 antibody
    • Popeye domain containing protein 2 antibody
    • Popeye protein 2 antibody
    see all

Anti-POPDC2 antibody images

  • All lanes : Anti-POPDC2 antibody (ab69176) at 1/500 dilution

    Lane 1 : POPDC2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-POPDC2 antibody (ab69176)

ab69176 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 10 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"