Overview

  • Product name
  • Description
    Rabbit polyclonal to PORCN
  • Tested applications
    Suitable for: WB, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 38-87 (ACRLLWRLGL PSYLKHASTV AGGFFSLYHF FQLHMVWVVL LSLLCYLVLF) of Human PORCN (NP_035050)

  • Positive control
    • HepG2 cell lysate ICC/IF: Panc-1 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab105543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-FoFr 1/300.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Modulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members.
  • Tissue specificity
    Isoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung.
  • Involvement in disease
    Defects in PORCN are the cause of focal dermal hypoplasia (FODH) [MIM:305600]; also known as Goltz Gorlin syndrome. A rare congenital ectomesodermal disorder characterized by a combination of skin defects, skeletal abnormalities, and ocular anomalies. Affected individuals have patchy dermal hypoplasia, often in a distribution pattern following the Blaschko lines, and areas of subcutaneous fat herniation or deposition of fat into the dermis. In addition, sparse and brittle hair, hypoplastic nails and papillomas have been described. Skeletal abnormalities usually comprise syndactyly, ectrodactyly, and brachydactyly, and in some cases osteopathia striata has been seen. Patients frequently have ocular anomalies, including microphthalmia/ anophthalmia, coloboma, pigmentary and vascularization defects of the retina. Dental abnormalities are often present.
  • Sequence similarities
    Belongs to the membrane-bound acyltransferase family. Porcupine subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Form
    Cellular component Endoplasmic reticulum, Membrane. Modulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members.
  • Alternative names
    • DHOF antibody
    • FODH antibody
    • MG61 antibody
    • MGC29687 antibody
    • por antibody
    • PORC antibody
    • PORCN antibody
    • PORCN_HUMAN antibody
    • PPN antibody
    • Probable protein-cysteine N-palmitoyltransferase porcupine antibody
    • Protein MG61 antibody
    see all

Images

  • Anti-PORCN antibody (ab105543) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 52 kDa
    12% SDS-PAGE
  • ab105543 staining PORCN in murine uterus tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
    Tissue was fixed in 4% PFA, blocked with 2% goat serum and then incubated with ab105543 at a 1/300 dilution at 4°C overnight. The secondary used was used at a 1/1000 dilution. Counterstain P.I. – 1/5000.
  • ICC/IF image of ab105543 stained Panc-1 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105543, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab105543 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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