Overview

  • Product nameAnti-Porimin antibody
    See all Porimin primary antibodies
  • Description
    Rabbit polyclonal to Porimin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to a region within C terminal amino acids 145-194 (SSVTITTTMH SEAKKGSKFD TGSFVGGIVL TLGVLSILYI GCKMYYSRRG) of Human Porimin (NP_443164).

  • Positive control
    • Placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

Target

  • RelevanceThis gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti PORIMIN (pro oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death.
  • Cellular localizationMembrane; Single pass type I membrane protein
  • Database links
  • Alternative names
    • KCT3 antibody
    • Keratinocytes associated transmembrane protein 3 antibody
    • Pro oncosis receptor inducing membrane injury antibody
    • Serine/threonine rich receptor antibody
    • TMEM123 antibody
    • Transmembrane protein 123 antibody
    see all

Anti-Porimin antibody images

  • Anti-Porimin antibody (ab81423) at 1 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References for Anti-Porimin antibody (ab81423)

ab81423 has not yet been referenced specifically in any publications.

Product Wall

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