• Product nameAnti-POT1 antibody
    See all POT1 primary antibodies
  • Description
    Rabbit polyclonal to POT1
  • Tested applicationsSuitable for: WB, ChIP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Pot1.

    (Peptide available as ab23432.)

  • Positive control
    • This antibody gave a positive signal in NIH3T3 and MEF1 whole cell lysates



Our Abpromise guarantee covers the use of ab21382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).
ChIP Use at an assay dependent concentration. PubMed: 24330541
ICC/IF 1/200.


  • FunctionComponent of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the telombin family.
  • Cellular localizationNucleus. Chromosome > telomere. Colocalizes with telomeric DNA.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP586D211 antibody
    • hPot 1 antibody
    • hPot1 antibody
    • POT 1 antibody
    • POT1 antibody
    • POT1 like telomere end-binding protein antibody
    • POT1 protection of telomeres 1 homolog antibody
    • POT1-like telomere end-binding protein antibody
    • POTE1_HUMAN antibody
    • Protection of telomeres 1 antibody
    • Protection of telomeres 1 homolog (S. pombe) antibody
    • Protection of telomeres protein 1 antibody
    see all

Anti-POT1 antibody images

  • All lanes : Anti-POT1 antibody (ab21382) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate with Human POT1 peptide (ab23432) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Predicted band size : 72 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
  • ab21382, at a 1/200 dilution, detecting Pot1 in methanol-fixed assynchronous Hela cells. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3; Counterstain: DAPI; Please refer to abreview for further details.

    See Abreview

  • All lanes : Anti-POT1 antibody (ab21382) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 72 kDa

References for Anti-POT1 antibody (ab21382)

This product has been referenced in:
  • Su CH  et al. An H2A Histone Isotype, H2ac, Associates with Telomere and Maintains Telomere Integrity. PLoS One 11:e0156378 (2016). ChIP ; Human . Read more (PubMed: 27228173) »
  • Hagiwara M  et al. POT1b regulates phagocytosis and NO production by modulating activity of the small GTPase Rab5. Biochem Biophys Res Commun 439:413-7 (2013). Read more (PubMed: 23954637) »

See all 8 Publications for this product

Product Wall

In theory the ab21382 should detect both Ms POT1a and POT1b but it isn't something we have specifically tested inhouse.

We'll gladly issue a credit note for the customer, but we need the PO or order number to do so.

Thank you for contacting us.

Ihavea question regarding the images:

The first lane is labeled as control: is this an untreated lysate or thecell line depleted for POT1?

The myc signal in lane three is quite weak, but nonet...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol

Dr. Kirk McManus

Verified customer

Submitted Apr 16 2007