Recombinant
RabMAb

Anti-POT1 antibody [EPR6319] (ab124784)

Overview

  • Product name
    Anti-POT1 antibody [EPR6319]
    See all POT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6319] to POT1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human POT1.

  • Positive control
    • K562, HepG2, 293T, and HeLa cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the telombin family.
    • Cellular localization
      Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZP586D211 antibody
      • hPot 1 antibody
      • hPot1 antibody
      • POT 1 antibody
      • POT1 antibody
      • POT1 like telomere end-binding protein antibody
      • POT1 protection of telomeres 1 homolog antibody
      • POT1-like telomere end-binding protein antibody
      • POTE1_HUMAN antibody
      • Protection of telomeres 1 antibody
      • Protection of telomeres 1 homolog (S. pombe) antibody
      • Protection of telomeres protein 1 antibody
      see all

    Images

    • All lanes : Anti-POT1 antibody [EPR6319] (ab124784) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 71 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Feng X  et al. CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance. Nucleic Acids Res 45:4281-4293 (2017). WB ; Human . Read more (PubMed: 28334750) »
    • Takai H  et al. A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus. Genes Dev 30:812-26 (2016). Read more (PubMed: 27013236) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LCL cells)
    Loading amount
    30 µg
    Specification
    LCL cells
    Gel Running Conditions
    Reduced Denaturing (10% SDS)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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