Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Use at an assay dependent concentration.
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
Expressed specifically in the neuroectodermal cell lineage.
Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
POU domain class 3 transcription factor 2 antibody
POU domain, class 3, transcription factor 2 antibody
POUF 3 antibody
Protein Brn 2 antibody
Western blot - POU3F2 antibody (ab94977)
All lanes : Anti-POU3F2 antibody (ab94977) at 1 µg/ml
Lane 1 : E16 Mouse Embryo Brain Tissue Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa Observed band size : 47 kDa Additional bands at : 200 kDa,35 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Immunocytochemistry/ Immunofluorescence - Anti-POU3F2 antibody (ab94977)This image is courtesy of an anonymous Abreview
ab94977 staining POU3F2 Figure A: Human immortalized Schwann cells transfected with mouse Brn2, fixed and stained 2 days post transfection under conditions specified in above review. Figure B: Non-transfected control under the same staining conditions by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS for 10 mins at 21°C and blocked with 5% normal Goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000 in 5% Goat serum) for 22 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-Rabbit IgG polyclonal (1/500) was used as the secondary antibody for 2 hours at 25°C.