Our Abpromise guarantee covers the use of ab94977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF Use at an assay dependent concentration.


  • FunctionTranscription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
  • Tissue specificityExpressed specifically in the neuroectodermal cell lineage.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain 2 antibody
    • Brain specific homeobox/POU domain protein 2 antibody
    • Brain-2 antibody
    • Brain-specific homeobox/POU domain protein 2 antibody
    • BRN 2 antibody
    • Brn-2 antibody
    • BRN2 antibody
    • N Oct 3 antibody
    • N OCT 3 Gene antibody
    • N Oct3 antibody
    • Nervous system specific octamer binding transcription factor antibody
    • Nervous system-specific octamer-binding transcription factor N-Oct-3 antibody
    • OCT 7 antibody
    • Oct-7 antibody
    • OCT7 antibody
    • Octamer Binding Transcription Factor 7 antibody
    • Octamer-binding protein 7 antibody
    • Octamer-binding transcription factor 7 antibody
    • OTF 7 antibody
    • OTF-7 antibody
    • OTF7 antibody
    • PO3F2_HUMAN antibody
    • POU class 3 homeobox 2, antibody
    • POU domain class 3 transcription factor 2 antibody
    • POU domain, class 3, transcription factor 2 antibody
    • Pou3f2 antibody
    • POUF 3 antibody
    • POUF3 antibody
    • Protein Brn 2 antibody
    see all

Anti-POU3F2 antibody images

  • All lanes : Anti-POU3F2 antibody (ab94977) at 1 µg/ml

    Lane 1 : E16 Mouse Embryo Brain Tissue Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 200 kDa,35 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ab94977 staining POU3F2 Figure A: Human immortalized Schwann cells transfected with mouse Brn2, fixed and stained 2 days post transfection under conditions specified in above review. Figure B: Non-transfected control under the same staining conditions by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS for 10 mins at 21°C and blocked with 5% normal Goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000 in 5% Goat serum) for 22 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-Rabbit IgG polyclonal (1/500) was used as the secondary antibody for 2 hours at 25°C.

    See Abreview

References for Anti-POU3F2 antibody (ab94977)

ab94977 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cortical neurons)
Specification cortical neurons
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Immortalized Schwann cells mouse Brn2 transfection)
Specification Immortalized Schwann cells mouse Brn2 transfection
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X100 10 min at room temperature
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 15 2012