The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 10 µg/ml.
FunctionMay play a role in determining or maintaining the identities of a small subset of visual system neurons.
Tissue specificityBrain. Seems to be specific to the retina.
Involvement in diseaseDefects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15) [MIM:602459]. DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Brain specific homeobox/POU domain protein 3C antibody
Brain-specific homeobox/POU domain protein 3C antibody
BRN 3C antibody
class 4 antibody
DFNA 15 antibody
POU class 4 homeobox 3 antibody
POU domain antibody
POU domain class 4 transcription factor 3 antibody
transcription factor 3 antibody
Anti-POU4F3 antibody images
Flow Cytometry - Anti-POU4F3 antibody (ab58128)
Overlay histogram showing HepG2 cells stained with ab58128 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58128, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - POU4F3 antibody (ab58128)
Predicted band size : 37 kDa POU4F3 antibody (ab58128) at 1ug/lane + HeLa cell lysate at 25ug/lane.