Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab58128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificityBrain. Seems to be specific to the retina.
  • Involvement in diseaseDefects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15) [MIM:602459]. DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox/POU domain protein 3C antibody
    • Brain-3C antibody
    • Brain-specific homeobox/POU domain protein 3C antibody
    • BRN 3C antibody
    • Brn-3C antibody
    • BRN3C antibody
    • class 4 antibody
    • DFNA 15 antibody
    • DFNA15 antibody
    • MGC138412 antibody
    • PO4F3_HUMAN antibody
    • POU class 4 homeobox 3 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 3 antibody
    • POU4F3 antibody
    • transcription factor 3 antibody
    see all

Anti-POU4F3 antibody images

  • Overlay histogram showing HepG2 cells stained with ab58128 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58128, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


  • Predicted band size : 37 kDa
    POU4F3 antibody (ab58128) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58128 at 10 ug/ml staining POU4F3 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-POU4F3 antibody (ab58128)

ab58128 has not yet been referenced specifically in any publications.

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