The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay 1/312500.
Use a concentration of 4 - 8 µg/ml.
FunctionMay play a role in determining or maintaining the identities of a small subset of visual system neurons.
Tissue specificityBrain. Seems to be specific to the retina.
Involvement in diseaseDefects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15) [MIM:602459]. DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Brain specific homeobox/POU domain protein 3C antibody
Brain-specific homeobox/POU domain protein 3C antibody
BRN 3C antibody
class 4 antibody
DFNA 15 antibody
POU class 4 homeobox 3 antibody
POU domain antibody
POU domain class 4 transcription factor 3 antibody
transcription factor 3 antibody
Anti-POU4F3 antibody images
Western blot - POU4F3 antibody (ab66260)
Anti-POU4F3 antibody (ab66260) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa Observed band size : 37 kDa Additional bands at : 34 kDa,45 kDa,64 kDa. We are unsure as to the identity of these extra bands.Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling POU4F3 with ab66260 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
References for Anti-POU4F3 antibody (ab66260)
This product has been referenced in:
Ishii J et al. Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation. Pathol Int64:415-22 (2014).
Read more (PubMed: 25243889) »
Walshe TE et al. TGF-ß signaling is required for maintenance of retinal ganglion cell differentiation and survival. Neuroscience189:123-31 (2011).
Read more (PubMed: 21664439) »