Overview

  • Product name
  • Description
    Rabbit polyclonal to POU6F1
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide derived from a region between amino acids 252-301 of human POU6F1. (Note: exact amino acid sequence is proprietary)

  • Positive control
    • Fetal Kidney Nuclear Lysate. Human intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab30944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 0.12 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Anti-POU6F1 antibody (ab30944) at 0.12 µg/ml (in 5% skim milk / PBS buffer) + Fetal Kidney Nuclear Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis human intestine tissue labelling POU6F1 with ab30944 at 4µg/ml. Arrows indicate positively stained epithelial cells of intestinal villus.

References

ab30944 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab30944.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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