Overview

  • Product namePP 1
  • Description
    Potent Src family tyrosine kinase inhibitor
  • Biological descriptionPotent Src family tyrosine kinase inhibitor. Inhibits a multitude of kinases (IC50 values are 0.05 (LCK), 0.52 (CSK), 0.64 (p38α) and 1.06 (CK1δ) µM). Inhibits Bcr-Abl kinase and induces apoptosis.
  • Purity> 98%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120859 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    PP 1 images

    • ab11512 staining E cadherin in SW480 cells treated with PP1 (ab120859), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of PP1, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120859 (PP1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for PP 1 (ab120859)

    This product has been referenced in:
    • Tatton L  et al. The Src-selective kinase inhibitor PP1 also inhibits Kit and Bcr-Abl tyrosine kinases. J Biol Chem 278:4847-53 (2003). Read more (PubMed: 12475982) »
    • Bain J  et al. The specificities of protein kinase inhibitors: an update. Biochem J 371:199-204 (2003). Read more (PubMed: 12534346) »
    • Hanke JH  et al. Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor. Study of Lck- and FynT-dependent T cell activation. J Biol Chem 271:695-701 (1996). Read more (PubMed: 8557675) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"