Overview

  • Product nameAnti-PP11 antibody
    See all PP11 primary antibodies
  • Description
    Rabbit polyclonal to PP11
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 116-253 of Human PP11 (P21128).

  • Positive control
    • Human placenta tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEndoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
  • Tissue specificityPlacental-specific, but also associated with various malignant neoplasms.
  • Sequence similaritiesBelongs to the ENDOU family.
    Contains 2 SMB (somatomedin-B) domains.
  • Post-translational
    modifications
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 22 serine protease antibody
    • 26 serine protease antibody
    • Endonuclease, polyU-specific antibody
    • endou antibody
    • ENDOU_HUMAN antibody
    • P11 antibody
    • Placental protein 11 antibody
    • Poly(U)-specific endoribonuclease antibody
    • PP11 antibody
    • Pp11r antibody
    • Protein endoU antibody
    • PRSS26 antibody
    • Uridylate-specific endoribonuclease antibody
    see all

Anti-PP11 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121367 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunocytochemical immunofluorescence analysis of methanol-fixed human HEK293T cells, labelling PP11 with 121367 at a dilution of 1/100 incubated for 16 hours at 4°C in 1% BSA in PBS with 0.1% Tween20 diluent. Blocking was with 1% BSA incubated for 1 hour at 25°C. Secondary was a goat anti-rabbit polyclonal Rhodamine conjugate at 1/100.

    See Abreview

  • ab121367 at 1/250 dilution staining PP11 in Paraffin-emebedded Human placenta tissue by Immunohistochemistry.

References for Anti-PP11 antibody (ab121367)

ab121367 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293T)
Permeabilization No
Specification HEK293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Methanol
Username

Mr. Chi-Cheng Hsieh

Verified customer

Submitted Nov 05 2015

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification HEK293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Chi-Cheng Hsieh

Verified customer

Submitted Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"