Overview

  • Product name
    Anti-PP14397 antibody
  • Description
    Rabbit polyclonal to PP14397
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from a region between residues 84-138 of Human PP14397.

  • Positive control
    • Jurkat cell lysate (western blot) and human skeletal muscle tissue slide (IHC-P).

Properties

Applications

Our Abpromise guarantee covers the use of ab22843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 5 µg/ml. Detects a band of approximately 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Images

  • ab22843 staining human muscle tissue sections by IHC-P.  The primary antibody was diluted 4 µg/ml.  Arrows indicate positively labelled skeletal muscle cells.

  • Anti-PP14397 antibody (ab22843) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti rabbit IgG

References

ab22843 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
30 µg
Specification
293T
Treatment
siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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