Overview

  • Product nameAnti-PP14397 antibody
    See all PP14397 primary antibodies
  • Description
    Rabbit polyclonal to PP14397
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human PP14397.

    (Peptide available as ab37970.)

  • Positive control
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

Anti-PP14397 antibody images

  • Anti-PP14397 antibody (ab36759) at 1 µg/ml + Jurkat whole cell lysate (ab7899) at 10 µl/ml buffer

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa

References for Anti-PP14397 antibody (ab36759)

ab36759 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa nuclear and cytoplasmic extract)
Loading amount 30 µg
Specification HeLa nuclear and cytoplasmic extract
Treatment JMJD8 specific siRNA
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Username

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Submitted Mar 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"