Overview

  • Product namePP2
  • Description
    Src and RIP2 kinase inhibitor
  • Biological descriptionInhibitor of the Src family of kinases. Inhibits Src and Lck kinase (IC50 values are 36 and 31 nM, respectively). Also shown to inhibit RIP2 and CK1δ (IC50 values are 19 and 41 nM, respectively).
  • Purity> 98%

Properties

  • Chemical name1-tert-Butyl-3-(4-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
  • Molecular weight301.78
  • Chemical structureChemical Structure
  • Molecular formulaC15H16ClN5
  • CAS Number172889-27-9
  • PubChem identifier4878
  • Storage instructionsStore at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overviewSoluble in DMSO to 50 mM and in ethanol to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESClc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C
  • Source

    Synthetic

  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab120308 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    PP2 images

    • ab11512 staining E-Cadherin in SW480 cells treated with PP2 (ab120308), by ICC/IF. Increase in E-cadherin expression correlates with increased concentration of PP2, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120308 (PP2) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab96971) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for PP2 (ab120308)

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"