Anti-PP2A alpha + beta antibody [1D6] (ab33537)

Overview

  • Product name
    Anti-PP2A alpha + beta antibody [1D6]
    See all PP2A alpha + beta primary antibodies
  • Description
    Mouse monoclonal [1D6] to PP2A alpha + beta
  • Specificity
    This antibody recognizes human PP2A, C subunit (PP2A alpha). It has been reported not to block phosphatase activity. The clone number has been updated from (0.T.118) to (1D6) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human, Xenopus laevis, Drosophila melanogaster
    Predicted to work with: Chicken, Dog, Pig, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human PP2A alpha + beta aa 295-309 (C terminal).
    Sequence:

    C-RGEPHVTRRTPDYFL

  • Positive control
    • WB: Human A431 and murine 3T3 RIPA cell lysates. IP: A431 RIPA lysate.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab33537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP Use a concentration of 4 µg/ml.
ICC Use a concentration of 5 - 10 µg/ml. Fix for 10 minutes with 4% paraformaldehyde in PBS.

Target

  • Function
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

References

This product has been referenced in:
  • Zuazua-Villar P  et al. Extensive RPA2 hyperphosphorylation promotes apoptosis in response to DNA replication stress in CHK1 inhibited cells. Nucleic Acids Res 43:9776-87 (2015). WB ; Human . Read more (PubMed: 26271993) »
  • Joseph BK  et al. Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d. J Biol Chem 290:10588-98 (2015). Read more (PubMed: 25694423) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam. Although we have eleven PP2A alpha antibodies, none of these have been raised in sheep and therefore none will be recognized by an anti-sheep IgG secondary antibody. SHould you decide to use ab33537 in your studies...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse adipocyte)
Loading amount
5 µg
Specification
mouse adipocyte
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 16 2011

Thank you for your enquiry. We currently have two PP2A C antibodies in our catalogue (ab1180, ab33537) and I have enquired at the originator of the product for more information. I have listed them below for your reference. ab1180 This product d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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