Recombinant
RabMAb

Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350)

Overview

  • Product name
    Anti-PP2A alpha + beta antibody [EPR11787(B)]
    See all PP2A alpha + beta primary antibodies
  • Description
    Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta
  • Host species
    Rabbit
  • Specificity
    The immunogen used for this product shares 100% homology with PP2A beta.
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PP2A alpha + beta.

  • Positive control
    • HeLa, 293T, A431 and NIH/3T3 cell lysates; A431 cells; Immunoprecipitation pellet from HeLa cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 36 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
    • Sequence similarities
      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Post-translational
      modifications
      Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • Cellular localization
      Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
    • Information by UniProt
    • Database links
    • Alternative names
      • PP2A A antibody
      • PP2A alpha antibody
      • PP2A B antibody
      • PP2A beta antibody
      • PP2A-alpha antibody
      • PP2AA_HUMAN antibody
      • PP2Ac antibody
      • PP2CB antibody
      • PPP2CA antibody
      • PPP2CB antibody
      • Protein phosphatase 2 catalytic subunit alpha isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isoform antibody
      • Replication protein C antibody
      • RP C antibody
      • RP-C antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
      see all

    Images

    • All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution

      Lane 1 : Human PP2A beta full length recombinant protein
      Lane 2 : Human PP2A alpha full length recombinant protein

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 59 kDa (why is the actual band size different from the predicted?)


      Exposure time: 15 seconds


      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

      Human PP2A beta full length recombinant protein contains aa1-309 with a GST-Tag (catalog#: ab128562).

      Human PP2A alpha full length recombinant protein contains aa1-309 with a GST-Tag (catalog#: ab128557).

    • All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunofluorescent analysis of A431 cells labeling PP2A alpha + beta with ab168350 at 1/50 dilution.

    • Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution + immunoprecipitation pellet from HeLa cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 36 kDa

    References

    ab168350 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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