The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.
Use a concentration of 0.5 - 1 µg/ml. In acetone fixed cell. Antigen retrieval by
Pepsin and Trypsin is required.
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Post-translational modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
Western blot - Anti-PP2A-alpha antibody (ab106262)
All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/ml
Lane 1 : Rat kidney tissue Lysate Lane 2 : Rat Liver tissue Lysate Lane 3 : Human Placenta tissue Lysate Lane 4 : Mouse Kidney tissue Lysate Lane 5 : A431 Whole Cell Lysate Lane 6 : HeLa Whole Cell Lysate Lane 7 : 293T Whole Cell Lysate Lane 8 : NIH Whole Cell Lysate Lane 9 : PC12 Whole Cell Lysate