Anti-PP2A alpha + beta antibody [0.T.118] (ab33537)

Overview

  • Product nameAnti-PP2A alpha + beta antibody [0.T.118]
    See all PP2A alpha + beta primary antibodies
  • Description
    Mouse monoclonal [0.T.118] to PP2A alpha + beta
  • SpecificityThis antibody recognizes human PP2A, C subunit (PP2A alpha). It has been reported not to block phosphatase activity.
  • Tested applicationsSuitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human, Xenopus laevis, Drosophila melanogaster
    Predicted to work with: Chicken, Dog, Pig, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human PP2A alpha + beta aa 295-309 (C terminal).
    Sequence:

    C-RGEPHVTRRTPDYFL

  • Positive control
    • WB: Human A431 and murine 3T3 RIPA cell lysates. IP: A431 RIPA lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP Use a concentration of 4 µg/ml.
ICC Use a concentration of 5 - 10 µg/ml. Fix for 10 minutes with 4% paraformaldehyde in PBS.

Target

  • FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2AA_HUMAN antibody
    • PP2Ac antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

References for Anti-PP2A alpha + beta antibody [0.T.118] (ab33537)

This product has been referenced in:
  • Ogawa H  et al. Protein phosphatase 2A negatively regulates aPKC signaling by modulating phosphorylation of Par-6 in Drosophila neuroblast asymmetric divisions. J Cell Sci 122:3242-9 (2009). IP ; Drosophila melanogaster . Read more (PubMed: 19690050) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam. Although we have eleven PP2A alpha antibodies, none of these have been raised in sheep and therefore none will be recognized by an anti-sheep IgG secondary antibody. SHould you decide to use ab33537 in your studies...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse adipocyte)
Loading amount 5 µg
Specification mouse adipocyte
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted May 16 2011

Thank you for your enquiry. We currently have two PP2A C antibodies in our catalogue (ab1180, ab33537) and I have enquired at the originator of the product for more information. I have listed them below for your reference. ab1180 This product d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"