The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesReactive with PP2AC from diverse tissue sources as determined by Western blotting. Used at 1:100 with bovine kidney cell extracts.
Not useful for immunoprecipitation.
Other immunodetection procedures not tested.
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Post-translational modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
Western blot - Anti-PP2A alpha + beta antibody (ab1180)
Western blot of 10 µg HEK293 cell extract with Anti-PP2A alpha. The band corresponds to the catalytic subunit of PP2A alpha (~ 36 kDa) Western blot of 10 µg HEK293 cell extract with Anti-PP2A alpha. The band corresponds to the catalytic subunit of PP2A alpha (~ 36 kDa)
References for Anti-PP2A alpha + beta antibody (ab1180)
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