Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065)

Overview

  • Product nameAnti-PP2A-(alpha + beta) antibody [YE351]
  • Description
    Rabbit monoclonal [YE351] to PP2A-(alpha + beta)
  • SpecificityThis antibody can bind both alpha and beta form of PP2A. Mouse samples have been successfully tested in WB, but IHC-P sections from various mouse tissues showed negative results.
  • Tested applicationsSuitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PP2A-(alpha + beta) (N terminal).

  • Epitopeab32065 reacts with an epitope located in the N terminal region of PP2A alpha.
  • Positive control
    • A431 cell lysate and NIH 3T3 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/80.
WB 1/500. Predicted molecular weight: 36 kDa.
IHC-P 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Anti-PP2A-(alpha + beta) antibody [YE351] images

    • All lanes : Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065) at 1/50000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : A549 whole cell lysate
      Lane 3 : Caco-2 whole cell lysate
      Lane 4 : T47D whole cell lysate
      Lane 5 : C2C12 whole cell lysate
      Lane 6 : HeLa whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 15 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065) at 1/50000 dilution

      Lane 1 : Jurkat whole cell lysate
      Lane 2 : U-937 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 5 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065) at 1/100000 dilution

      Lane 1 : Human fetal heart tissue lysate
      Lane 2 : Human fetal kidney tissue lysate
      Lane 3 : Human fetal spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 30 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065) at 1/100000 dilution + Human fetal brain tissue lysate at 10 µg

      Secondary
      HRP-conjugate anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 15 seconds

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065) at 1/100000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse kidney tissue lysate
      Lane 4 : Mouse spleen tissue lysate
      Lane 5 : Rat brain tissue lysate
      Lane 6 : Rat heart tissue lysate
      Lane 7 : Rat kidney tissue lysate
      Lane 8 : Rat spleen tissue lysate
      Lane 9 : C6 whole cell lysate
      Lane 10 : Raw264.7 whole cell lysate
      Lane 11 : PC-12 whole cell lysate
      Lane 12 : NIH/3T3 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 1 second

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PP2A-(alpha + beta) with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasm and weak nucleus staining is visible in lymphocytes of human tonsil.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling PP2A-(alpha + beta) with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasm staining is visible in epithelial cells of mouse kidney

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling PP2A-(alpha + beta) with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasm and weak nucleus staining is visible in epithelial cells of rat kidney.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling PP2A-(alpha + beta) with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasm staining is visible in mouse heart.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat heart tissue labelling PP2A-(alpha + beta) with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasm and nucleus staining is visible on rat heart.

    • ab32065 at 1/80 immunoprecipitating PP2A-(alpha + beta) in HeLa whole cell lysate.

      Lane 1 (input): HeLa whole cell lysate (10µg)

      Lane 2 (+): ab32065 + HeLa whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32065 in HeLa (human cervix adenocarcinoma) whole cell lysate.

      For western blotting, ab32065 (1/500) ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      Observed band: 37kDa.

       

    • Dot blot analysis of PP2A-alpha peptide (Lane 1) and PP2A-beta peptide (Lane 2) labelling PP2A-(alpha+beta) with ab32065 at a dilution of 1/1000. A HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at a dilution of 1/1000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    References for Anti-PP2A-(alpha + beta) antibody [YE351] (ab32065)

    This product has been referenced in:
    • Langlois B  et al. LRP-1 promotes cancer cell invasion by supporting ERK and inhibiting JNK signaling pathways. PLoS One 5:e11584 (2010). WB . Read more (PubMed: 20644732) »

    See 1 Publication for this product

    Product Wall

    Thank you for your patience in awaiting our reply while we gathered our data on this. It does not appear that this has been specifically tested for our products but comparing the immunogens of our PP2CA antibodies there is a strong possibility that eac...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"