Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597)
Key features and details
- Mouse monoclonal [2A10] to PP2A alpha + beta (methyl L309)
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-PP2A alpha + beta (methyl L309) antibody [2A10]
See all PP2A alpha + beta primary antibodies -
Description
Mouse monoclonal [2A10] to PP2A alpha + beta (methyl L309) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Saccharomyces cerevisiae -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Hek293 and PC12 whole cell lysates
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2A10 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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Notes |
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WB
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 24672 Rat
- Entrez Gene: 24673 Rat
- Omim: 176915 Human
- Omim: 176916 Human
see all -
Alternative names
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
see all
Images
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All lanes : Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 20 minutes -
All lanes : Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 1/1000 dilution
Lane 1 : No LCMT1 with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Lane 2 : LCMT1 at 0.02 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Lane 3 : LCMT1 at 0.1 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Lane 4 : LCMT1 at 0.5 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Secondary
All lanes : IRDye 800CW conjugated goat anti-mouse polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Detection method: Licor 10 minute exposure.
Specific observed bands at 40kDa
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab66597 has been referenced in 13 publications.
- Liang Y et al. PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production. Cell Death Differ 28:2728-2744 (2021). PubMed: 33934104
- Wu Z et al. Juvenile hormone acts through FoxO to promote Cdc2 and Orc5 transcription for polyploidy-dependent vitellogenesis. Development 147:N/A (2020). PubMed: 32907849
- Lin DW et al. Microhomology-based CRISPR tagging tools for protein tracking, purification, and depletion. J Biol Chem 294:10877-10885 (2019). PubMed: 31138654
- Chen XY et al. LB100 ameliorates nonalcoholic fatty liver disease via the AMPK/Sirt1 pathway. World J Gastroenterol 25:6607-6618 (2019). PubMed: 31832001
- Wei Y et al. Ribosylation triggering Alzheimer's disease-like Tau hyperphosphorylation via activation of CaMKII. Aging Cell 14:754-63 (2015). WB ; Mouse . PubMed: 26095350