Overview

  • Product nameAnti-PP2A beta antibody
    See all PP2A beta primary antibodies
  • Description
    Rabbit polyclonal to PP2A beta
  • Specificityab32673 recognises PP2A beta. Does not crossreact with PP1 or other protein phosphatases.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Chicken, Cow, Dog, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Cynomolgus monkey, Dictyostelium discoideum
  • Immunogen

    Synthetic peptide corresponding to Human PP2A beta aa 296-309 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    GEPHVTRRTPDYFL

  • Positive control
    • Rabbit brain membrane cytosol and human A431 RIPA cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.
IP Use a concentration of 4 - 5 µg/ml.

Target

  • FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
  • Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP2A beta antibody
    • PP2A-beta antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2CB antibody
    • Ppp2cb antibody
    • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isozyme antibody
    • Protein phosphatase 2, catalytic subunit, beta isoform antibody
    • Protein phosphatase 2A catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
    see all

References for Anti-PP2A beta antibody (ab32673)

ab32673 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"