Overview

  • Product nameAnti-PP5 antibody
    See all PP5 primary antibodies
  • Description
    Rabbit polyclonal to PP5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide derived from within residues 150 - 250 of Human PP5.

    (Peptide available as ab27751.)

  • Positive control
    • This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab34693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human PP5 peptide (ab27751).

Target

  • FunctionMay play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
    Contains 3 TPR repeats.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36922 antibody
    • FLJ55954 antibody
    • OTTHUMP00000165834 antibody
    • PP-T antibody
    • PP5 antibody
    • PPP5 antibody
    • PPP5_HUMAN antibody
    • Ppp5c antibody
    • PPT antibody
    • Protein phosphatase 5, catalytic subunit antibody
    • Protein phosphatase T antibody
    • Serine/threonine protein phosphatase 5 antibody
    • Serine/threonine-protein phosphatase 5 antibody
    see all

Anti-PP5 antibody images

  • All lanes : Anti-PP5 antibody (ab34693) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa (possible cross reactivity).

References for Anti-PP5 antibody (ab34693)

This product has been referenced in:
  • Tukaj S  et al. Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. PLoS One 8:e70496 (2013). Read more (PubMed: 23936217) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Sample Rat Tissue lysate - other (Spinal cord)
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Loading amount 60 µg
Specification Spinal cord
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Emiliya Storman

Verified customer

Submitted Jun 15 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT1080 Cell line)
Specification HT1080 Cell line
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 04 2010

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount 50000 cells
Specification HT1080 Cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted May 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"