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Rabbit polyclonal to PP5
Predicted to work with:
Rat, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
A synthetic peptide corresponding to a region between residue 1 and 50 of human PP5 (NP_006238.1).
Whole cell lysate from Hela cells, 293T cells and NIH3T3 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Use at 2-5 µg/mg of lysate.
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats.
Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP5 antibody (ab71150)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PPP5 with ab71150 at 1/200 (1µg/ml). Detection: DAB.
Western blot - PP5 antibody (ab71150)
All lanes :
Anti-PP5 antibody (ab71150) at 0.04 µg/ml
Lane 1 :
Whole cell lysate from Hela cells at 50 µg
Lane 2 :
Whole cell lysate from Hela cells at 15 µg
Lane 3 :
Whole cell lysate from Hela cells at 5 µg
Lane 4 :
Whole cell lysate from 293T cells at 50 µg
Lane 5 :
Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size:
Observed band size:
60 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - PP5 antibody (ab71150)
Immunoprecipitation/ Western Blot of PP5
Lane 1: ab71150 at 3µg/mg whole cell lysate.
Lane 2: IgG control IP
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Subsequent WB detection was performed using 1 µg/ml ab71150.
Chemiluminescence with an exposure time of 5 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"