Anti-PPAN antibody (ab122844)
Key features and details
- Rabbit polyclonal to PPAN
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPAN antibody -
Description
Rabbit polyclonal to PPAN -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: RWEMDRGRGR LCDQKFPKTK DKSQGAQARR GPRGASRDGG, corresponding to amino acids 421-460 of Human PPAN.
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Positive control
- RT4, U-251 MG and Human Tonsil lysates, Human oral mucosa tissue, U-251 MG cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
May have a role in cell growth. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 Brix domain. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 56342 Human
- Omim: 607793 Human
- SwissProt: Q9NQ55 Human
- Unigene: 14468 Human
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Alternative names
- Brix domain containing protein 3 antibody
- Brix domain-containing protein 3 antibody
- BXDC3 antibody
see all
Images
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All lanes : Anti-PPAN antibody (ab122844) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
ab122844 at 1/200 dilution staining PPAN in Paraffin-embedded Human oral mucosa tissue by Immunohistochemistry.
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Immunofluorescent staining of Human cell line U-251 MG using ab122844 at a concentration of 4 µg/ml. ab122844 staining is shown in green.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122844 has been referenced in 1 publication.
- Keil M et al. Loss of Peter Pan protein is associated with cell cycle defects and apoptotic events. Biochim Biophys Acta Mol Cell Res 1866:882-895 (2019). PubMed: 30716409