• Product name
  • Description
    Rabbit polyclonal to PPAP2A
  • Specificity
    The immunogen for ab58745 is derived from a region specifically from isoform 2. This sequence shows low homology with isoform 1.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Guinea pig, Cat
  • Immunogen

    Synthetic peptide within Human PPAP2A aa 31-80 (N terminal). The exact sequence is proprietary. Isoform 2


  • Positive control
    • HepG2 cell lysate or human kidney tissue.



Our Abpromise guarantee covers the use of ab58745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.


  • Function
    Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). Pivotal regulator of lysophosphatidic acid (LPA) signaling in the cardiovascular system. Major enzyme responsible of dephosphorylating LPA in platelets, which terminates signaling actions of LPA. May control circulating, and possibly also regulate localized, LPA levels resulting from platelet activation. It has little activity towards ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > S-1-P > C-1-P. It's down-regulation may contribute to the development of colon adenocarcinoma.
  • Tissue specificity
    Ubiquitously expressed with highest expression found in prostate. Isoform 1 is predominant in kidney, lung, placenta and liver. Isoform 2 is predominant in heart and pancreas. Found to be down-regulated in colon adenocarcinomas.
  • Sequence similarities
    Belongs to the PA-phosphatase related phosphoesterase family.
  • Post-translational
    N-glycosylated. Contains high-mannose oligosaccharides.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid phosphate phosphohydrolase 1 antibody
    • Lipid phosphate phosphohydrolase 1a antibody
    • LLP1a antibody
    • LPP1 antibody
    • LPP1_HUMAN antibody
    • PAP 2a antibody
    • PAP-2a antibody
    • PAP2 antibody
    • PAP2-alpha antibody
    • PAP2a antibody
    • PAP2a2 antibody
    • PAP2alpha2 antibody
    • PAPalpha1 antibody
    • Phosphatidate phosphohydrolase type 2a antibody
    • Phosphatidic acid phosphatase 2a antibody
    • Phosphatidic acid phosphatase type 2A antibody
    • Phosphatidic acid phosphohydrolase type 2a antibody
    • PPAP2A antibody
    • Type 2 phosphatidic acid phosphatase alpha antibody
    • Type 2 phosphatidic acid phosphohydrolase antibody
    see all

Anti-PPAP2A antibody images

  • Anti-PPAP2A antibody (ab58745) at 5 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPAP2A with ab58745 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-PPAP2A antibody (ab58745)

This product has been referenced in:
  • Evseenko D  et al. Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironment. PLoS One 8:e63718 (2013). IHC-P ; Human . Read more (PubMed: 23696850) »

See 1 Publication for this product

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