Overview

  • Product nameAnti-PPAR alpha antibody [1E8]
    See all PPAR alpha primary antibodies
  • Description
    Mouse monoclonal [1E8] to PPAR alpha
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 145-420 of Human PPAR alpha.

  • Positive control
    • HEPG2 lysate, SVT2 lysate.

Associated products

Applications

Our Abpromise guarantee covers the use of ab119416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 52.2 kDa.
ICC/IF 1/100.

Target

  • FunctionLigand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificitySkeletal muscle, liver, heart and kidney.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Anti-PPAR alpha antibody [1E8] images

  • All lanes : Anti-PPAR alpha antibody [1E8] (ab119416)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control lysate
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PPARA lysate

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52.2 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216176)
  • All lanes : Anti-PPAR alpha antibody [1E8] (ab119416)

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 52.2 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216176)
  • ab119416, staining PPAR alpha by immunocytochemistry on COS7 cells transiently transfected with pCMV6-ENTRY PPARA.

References for Anti-PPAR alpha antibody [1E8] (ab119416)

ab119416 has not yet been referenced specifically in any publications.

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