Anti-PPAR alpha antibody (ab126285)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PPAR alpha
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
    Predicted to work with: Rat, Cow, Dog, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human PPAR alpha.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; NIH3T3; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab126285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificity
    Skeletal muscle, liver, heart and kidney.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Images

  • All lanes : Anti-PPAR alpha antibody (ab126285) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References

ab126285 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Rabbit Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 25 2013

Perdona por la espera.

He estado consultando las posibilidades de reaccionar con lisados celulares de conejos entre los anticuerpos contra PPAR alpha que habíamos comentado.

Tengo buenas noticias, pues varios de ellos muestra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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