Anti-PPAR alpha antibody (ab61182)

Overview

  • Product nameAnti-PPAR alpha antibody
    See all PPAR alpha primary antibodies
  • Description
    Rabbit polyclonal to PPAR alpha
  • Specificityab61182 detects PPAR alpha protein, (SwissProt Q07869).
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human PPAR alpha around the phosphorylation site of serine 21 (L-E-SP-P-L).

  • Positive control
    • NIH 3T3 cell extracts and HeLa cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab61182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionLigand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificitySkeletal muscle, liver, heart and kidney.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Anti-PPAR alpha antibody images

  • ab61182 at 1/100 - 1/500 dilution staining PPAR alpha in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-PPAR alpha antibody (ab61182) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extracts
    Lane 2 : NIH 3T3 cell extracts with the immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-PPAR alpha antibody (ab61182)

ab61182 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab846.
To check th...

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Thank you for your reply.
For PPAR alpha, I would recommend ab27478 as an isotype control.
http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html

As for the CD4 antibody, your protocol seems to be fine. The immunogen sequence is l...

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Thank you for contacting us.
To answer your questions:
1. Yes, a negative control can be ommiting the primary. Other options could be using an isotype control antibody that compares with the priamry, or the blocking peptide. There are various...

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Yes, but none that are raised against an intracellular domain. For instance, ab13438 is raised against an intact cell preparation. The clone, Q4120, blocks the receptor, confirming that the epitope it recognizes is extracellular. http://www.abcam.com/i...

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Thank you for contacting us. The antibodies I recommend are: PPAR alpha rabbit polyclonal - ab61182 Secondary - goat anti-rabbit IgG Dylight 488-conjugated, pre-adsorbed ab96899 CD4 mouse monoclonal - ab25804 Secondary - rat anti-mouse IgG1 PE-co...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (Expression of Human PPAR Alpha in CHO)
Specification Expression of Human PPAR Alpha in CHO
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3%
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Submitted Jul 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"