Anti-PPAR alpha antibody [OTI1E8] (ab119416)

Overview

  • Product name
    Anti-PPAR alpha antibody [OTI1E8]
    See all PPAR alpha primary antibodies
  • Description
    Mouse monoclonal [OTI1E8] to PPAR alpha
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 145-420 of Human PPAR alpha.

  • Positive control
    • HEPG2 lysate, SVT2 lysate.
  • General notes

    The clone number has been updated from 1E8 to OTI1E8, both clone numbers name the same clone.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Applications

Our Abpromise guarantee covers the use of ab119416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 52.2 kDa.
ICC/IF 1/100.

Target

  • Function
    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificity
    Skeletal muscle, liver, heart and kidney.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Images

  • All lanes : Anti-PPAR alpha antibody [OTI1E8] (ab119416)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control lysate
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PPARA lysate

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52.2 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216176)
  • All lanes : Anti-PPAR alpha antibody [OTI1E8] (ab119416)

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 52.2 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216176)
  • ab119416, staining PPAR alpha by immunocytochemistry on COS7 cells transiently transfected with pCMV6-ENTRY PPARA.

References

ab119416 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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