Recombinant
RabMAb

Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)

Overview

  • Product name
    Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)]
  • Description
    Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PPAR alpha + PPAR beta/delta aa 1-200. The exact sequence is proprietary.
    Database link: Q03181

  • Positive control
    • WB: Human PPAR alpha and delta full length recombinant proteins; Human fetal liver, fetal heart and fetal kidney lysates; Human skeletal muscle lysate; Jurkat, HeLa, K562, HepG2, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse placenta, brain, heart, kidney and spleen lysates; Rat placenta, brain, heart and kidney lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16673(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 52, 50 kDa).

Images

  • All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution

    Lane 1 : Human PPAR alpha full length recombinant protein
    Lane 2 : Human PPAR beta full length recombinant protein
    Lane 3 : Human PPAR gamma fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.

    Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.

  • All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 5 : Mouse placenta lysate
    Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : Rat placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3,4 and 5: 2 seconds; Lane 6: 1 second; Lane 7: 30 seconds.

  • All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Mouse brain lysate at 10 µg
    Lane 2 : Mouse heart lysate at 10 µg
    Lane 3 : Mouse kidney lysate at 10 µg
    Lane 4 : Mouse spleen lysate at 10 µg
    Lane 5 : Rat brain lysate at 10 µg
    Lane 6 : Rat heart lysate at 10 µg
    Lane 7 : Rat kidney lysate at 10 µg
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
    Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
    Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3 and 4: 2 seconds; Lane 5,6,7,8,9,10 and 11: 1 second.

References

ab178865 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab178865.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up